As much as I portray labwork as a fun experience (which it is), it can be extremely brain numbing. This week stood on the more busy side with multiple experiments back to back (flows and westerns) and burning my eyes staring at my data trying to understand it. It’s made feel like me flipping tables like my friend Herbert–>
Some issues we ran into for my experiments was (1) unspecific binding in my westerns, meaning there were too much bands that showed up making it hard for me to detect the bands we actually want and (2) no binding from my antibodies for flow. After a retry for the flow experiment and some troubleshooting, the flow worked as normal. (There are actually lots of long papers on PubMed just dedicated to troubleshooting all the ways a western can go wrong!) As of now the western has yet to be “fixed”, but I’m sure with some problem solving it will be up and running like normal.
On the contrary, this week was also the week for summer intern candidates! It was exciting to meet and talk to all of them, a nice break from data analysis. It was interesting to watch the research version of The Bachelor, like “Whoever shall we pass the pipette to?”
Forever grateful for reality tv and prescription glasses,