The One with the Graphs

Apr 07, 2019

At this point in the project, all my lab procedures were completed. The Alizarin Stain was used to measure calcification in the aortic valve, echocardiography was used to measure functionality, and immunohistological analysis was used to measure pro-osteogenic tendencies. With all this data, I needed to figure out what it meant.

I created six different graphs:

  1. Effects of SIRT6 reduction on Calcium Burden in Young Mice
  2. Effects of SIRT6 reduction on Calcium Burden in Old Mice
  3. Effects of SIRT6 reduction on Aortic Valve Function in Young Mice
  4. Effects of SIRT6 reduction on Aortic Valve Function in Old Mice
  5. Effects of SIRT6 reduction on Sp7 Protein Expression in Young Mice
  6. Effects of SIRT6 reduction on Aortic Valve Function in Old Mice

Each of these graphs contained a WT and Cre+ bar. This method of displaying the data allowed me to easily visualize the differences created by the reductions of SIRT6. I also added error bars to the graphs, which let me know whether the data would be statistically relevant or not. Using my graphs, I created a scientific research poster, which concluded my time at the lab in Rochester.

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