There are a lot of aspects of my research that keep me waiting. Waiting for public transportation, waiting for countless centrifuge spinnings, and ..waiting for antibodies to arrive in the mail. This week what I have been doing has been mostly the last one. It started off researching the different antibodies and their functions to searching for those spicy deals on the more cost effective antibodies for my project. This process actually took a long time, because the antibodies were so expensive! (A small tube of maybe 25~ microliters could cost around $100+ dollars) After ordering them, I ended up just doing a lot of computer related work, so not much wet labwork. Another activity I ended up doing while waiting for my antibodies, brushing up on biology. It has been a while since I have had a sit down lecture, and my lab mates helped teach me the science behind techniques etc.Speaking of techniques, another one I learned about and finally did, western blotting! More on that later. (Things I have been doing in between spare time, catching up on reading 😀 . It’s been a quite a while since school ended, and my brain hasn’t been stimulated with large blocks of text in a long time, so I’ve picked up some fiction books from the library to read.)
This week marks the first official kickoff for my senior project, and I finally want to introduce my project. In essence, from previous research my mentor has found some proteins that play significant roles in cell signaling as surface receptors. What I am doing is taking those list of proteins, and testing how Ikaros affects how they are expressed on the RNA level. Which is why I will be using flow cytometry- detecting physical and chemical characteristics in a population- and western blotting – detecting specific proteins- in my project!
It’s been a long process, but what I gained from it was an essential trait: more patience.